CPSA Brasil 2014

Emerging Models for Drug Development and the Clinical Laboratory: Innovative Technologies and Future Applications

2014 Plenary Speaker

2014 Plenary Speaker
Shane Needham
Alturas Analytics, Inc.

Shane Needham, Ph.D.

Microspray and Microflow Liquid Chromatography: The Perfect Fit for Modern LC-MS Bioanalyis

The predominant analytical technique of choice in bioanalytical labs is HPLC-MS/MS for the determination of drugs and metabolites from biological fluids. Typically, these instruments use variances of electropspray ionization (ESI) with HPLC flow rates of 100-700 µL/minute. As is well known, the relationship between LC flow rate and LC-MS sensitivity shows that operation of the LC at lower flow rates can deliver sensitivity benefits. This is well known in the proteomics community, where scientists routinely use nanoHPLC-MS techniques for sensitivity. Now that vendors can produce microflow LC (MFLC) equipment that deliver accurate and precise flows at low µL/minute rates, the bioanalytical community sees the benefit in operating under these parameters. Additional gains such as lower required sample volumes, solvent consumption and reduced source contamination make MFLC-MS/MS even more attractive to the bioanalytical lab. At these µL/minute flow rates, the ESI-MS source can be coupled with the MFLC column to further improve MS signal and chromatography compared to traditional segregated techniques. As we see advantages of MFLC in the application of limited volume bioanalytical samples and the benefits of microspray coupled with LC-MS, we understand why, “microspray and MFLC are the perfect fit for the future of bioanalysis”.

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